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KMID : 1094719970020020097
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Biotechnology and Bioprocess Engineering
1997 Volume.2 No. 2 p.97 ~ p.100
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Production of cyclodextrin homologues using aqueous two-phase system
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Chang Woo-Jin
Koo Yoon-Mo
Park Sung-Sik
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Abstract
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Cyclodextrin homologues (CDs), produced by cyclodextrin glycosyltransferase (CGTase), were simultaneously partitioned in aqueous two-phase system (ATPS). Partition coefficients of CDs were measured in PEG/salt and PEG/dextran systems. Phosphate, citrate, sulfate were tested as salt. ATPS of PEG/salt and PEG/dextran had the partition coefficients of the CDs, larger than unity. However, PEG/dextran system was observed better than PEG/salt as CGTase activity decreased sharply with salt concentration. Enzymatic reaction occurred mainly in PEG-rich bottom phase because of the low partition coefficient of CGTase. The resulting CDs transferred to the PEG-rich top phase, obeying the diffusional partition. In the ATPS of 7% PEG (M.W. 20,000) and 9% dextran (M.W. 40,000), 7 mg/ml of CDs were obtained in top phase at 4.5 hours.
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KEYWORD
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cyclodextrin homologues, aqueous two-phase system, simultaneous production and separation, CGTase
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